BPW-HQ enrichment broth for Neogen Salmonella One-Broth-One-Plate ISO16140 validated method

NEOGEN has developed an innovative workflow solution for the detection of pathogens in food and environmental samples, using just one enrichment broth and one agar plate. Ideal for laboratories looking to reduce time to result whilst still maintaining classical microbiology, One-Broth-One-Plate (OBOP) workflows have increased sensitivity versus their corresponding ISO methods, with results in as little as 48 hours. Increased sensitivity is achieved through each workflow’s proprietary enrichment step. OBOP workflows, available for the detection of Listeria spp., Listeria monocytogenes and Salmonella spp., have been independently validated and certified according to ISO 16140-2, as an alternative testing method to the associated ISO reference methods. One-Broth-One-Plate for Salmonella (OBOP-S) offers a rapid method for the enrichment and detection of Salmonella spp. using traditional culture methodology. One-Broth-One-Plate for Salmonella utilises Neogen’s BPW-HQ for a single overnight enrichment step designed to recover damaged Salmonella. The formulation of the base media is compliant to ISO 6579-1:2017. When used in conjunction with the OBOP-S Selective Supplement, Buffered Peptone Water constitutes a selective medium for an alternative workflow to ISO 6579-1:2017. Salmonella Selective Supplement is provided separately to enable the common use of the medium as a diluent for other analyses from that sample prior to overnight incubation. Detection is via Chromogenic agar for Salmonella Esterase (CASE), a selective chromogenic agar for the detection of Salmonella. The broth is available as a dehydrated powder or ready-to-use in bags and 225ml bottles.

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