PATHfinder – 4-Plex Real-Time PCR kit for the detection of foodborne STEC based on ISO13136 and EFSA – [stx1]/[stx2 + stx2f]/[eae]/IAC
- Format: 50 Tests
- Code: PMB10A-EFSA-50
- Technology: Real-Time PCR
- Application: Food/Water microbiology
- Tag/Filter: Cytotoxic E.coli
PATHfinder Real-Time PCR kits are primary tools for modern food and water quality and safety testing. The kits detect in different sample the presence and the identity of microrganisms through their DNA.The kits are developed according to the norms ISO22119, ISO20838, ISO16140 hence they include an internal amplification control (IAC), a positive and a negative control. This kit is based on ISO13136 indications and when paired to an appropriate DNA extraction kit (e.g. Generon FASTfood - Cat# EXD009) it allows for the detection of STEC in less than 2 hours. STEC can be detected in enriched media broth or in water samples collection filters. The kit in less than 30 minutes, without any DNA extraction step, allows also for the identification as STEC of a colony isolated on a plated growth medium. The kit contains a 4-plex PCR mix for 50 tests targeting: stx1 (FAM) - IAC (HEX) - stx2+stx2f (TxRed) - eae (Cy-5)
- Format: 50 Tests
- Code: PMB10A-EFSA-50
- Technology: Real-Time PCR
- Application: Food/Water microbiology
- Tag/Filter: Cytotoxic E.coli
