PATHfinder – Real-Time PCR kit for STEC Big 7 serotyping – [O157, O111, O26, O103, O145, O121, O45] prealiquoted
- Format: 12 Strips
- Code: PMB10A-OST
- Technology: Real-Time PCR
- Application: Food/Water microbiology
- Tag/Filter: Cytotoxic E.coli
PATHfinder Real-Time PCR kits are primary tools for modern food and water quality and safety testing. The kits detect in different sample the presence and the identity of microrganisms through their DNA.The kits are developed according to the norms ISO22119, ISO20838, ISO16140 hence they include an internal amplification control, a positive and a negative control. This kit is based on ISO13136 indications and when paired to an appropriate DNA extraction kit (e.g. Generon FASTfood - Cat# EXD009) it allows for the detection of the so called Big7 STEC (namely O157, O111, O26, O103, O145, O121, O45) in less than 2 hours. STEC positives can be detected in enriched media broth or in water samples collection filters. The kit in less than 30 minutes, without any DNA extraction step, allows also for the identification as STEC positive of a colony isolated on a plated growth medium. The kit contains seven ready-to-use 2-plex PCR mixes conveniently pre-aliquoted in 8-wells PCR strips: MIX1 [O157 (FAM) IAC (HEX)] - MIX2 [O111 (FAM) IAC (HEX)] - MIX3 [O26 (FAM) IAC (HEX)] - MIX4 [O103 (FAM) IAC (HEX)] - MIX5 [O145 (FAM) IAC (HEX)] - MIX6 [O121 (FAM) IAC (HEX)] - MIX7 [O45 (FAM) IAC (HEX)].
- Format: 12 Strips
- Code: PMB10A-OST
- Technology: Real-Time PCR
- Application: Food/Water microbiology
- Tag/Filter: Cytotoxic E.coli
